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KMID : 0390119930330020177
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Journal of Pusan Medical College
1993 Volume.33 No. 2 p.177 ~ p.186
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Effect of Dexamethasone on Cellular Functions in Rabbit Brain Slices under Hypoxia
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Abstract
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The effect of dexamethasone on cellular electrolytes an water content, lipid peroxidation, K-dependent p-phenylphosphatase (k-pNPPase) activity in rabbit brain slices and Na-K-ATPase activity in microsome were studied and the results are as
follows.
1. Under 100% N2-environment rabbit brain slices showed accumulation of Na and water, and decrease of K by 36%, 312% and 51% of control value after 2 hours, respectively. Dexamethasone (1 mM) inhibited these changes by hypoxia, but had no
significant
effect under 100% O2-environment.
2. Microsomal Na-K-ATPase activity was not influenced by addition of 1 mM dexamethasone, but k-pNPPase activity in slices decreased by 100% N2-environment was significantly recovered by dexamethasone.
3. Lipid peroxidation was enhanced under 100% N2-environment and this increase was significantly blocked by 1 mM dexamethasone.
4. Under existence of 1 mM ouabain, dexamethasone still showed blocking effect on ouabain-induced changes of electrolytes and water content.
Above results indicate that dexamethasone exerts its stabilizing effect on electrolytes and water contents of rabbit brain slices under hypoxia by inhibiting lipid peroxidation, thereby enhancing Na-pump activity and also by decreasing the
membrane
permeability to electrolytes and water.
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